| Class 1A - Region R3 - District D5 | |||
|---|---|---|---|
| School | City | ||
| Andrew Jackson (Jacksonville) | Jacksonville | ||
| Bolles (Jacksonville) | Jacksonville | ||
| Cedar Creek Christian (Jacksonville) | Jacksonville | ||
| Episcopal (Jacksonville) | Jacksonville | ||
| Fernandina Beach | Fernandina Beach | ||
| University Christian (Jacksonville) | Jacksonville | ||
| West Nassau (Callahan) | Callahan | ||
| Wolfson (Jacksonville) | Jacksonville | ||
| Class 1A - Region R3 - District D6 | |||
| School | City | ||
| Baldwin | Baldwin | ||
| Bishop Snyder (Jacksonville) | Jacksonville | ||
| Bradford (Starke) | Starke | ||
| Eagles View (Jacksonville) | Jacksonville | ||
| Fort White | Fort White | ||
| Keystone Heights | Keystone Heights | ||
| St. Johns Country Day (Orange Park) | Orange Park | ||
| Union County (Lake Butler) | Lake Butler | ||
| Class 1A - Region R4 - District D7 | |||
| School | City | ||
| Bell | Bell | ||
| Branford | Branford | ||
| Bronson | Bronson | ||
| Cedar Key | Cedar Key | ||
| Chiefland | Chiefland | ||
| Newberry | Newberry | ||
| Trenton | Trenton | ||
| Williston | Williston | ||
| Class 1A - Region R4 - District D8 | |||
| School | City | ||
| Crescent City | Crescent City | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Florida Deaf and Blind (St. Augustine) | St. Augustine | ||
| Hawthorne | Hawthorne | ||
| Interlachen | Interlachen | ||
| Palatka | Palatka | ||
| Taylor (Pierson) | Pierson | ||
| Class 1A - Region R5 - District D9 | |||
| School | City | ||
| Hernando Christian (Brooksville) | Brooksville | ||
| Mount Dora Christian | Mount Dora | ||
| South Sumter (Bushnell) | Bushnell | ||
| St. John Lutheran (Ocala) | Ocala | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Umatilla | Umatilla | ||
| Villages | Middleton | ||
| Wildwood | Wildwood | ||
| Class 1A - Region R5 - District D10 | |||
| School | City | ||
| Cornerstone Charter (Belle Isle) | Belle Isle | ||
| Faith Christian (Orlando) | Orlando | ||
| First Academy (Orlando) | Orlando | ||
| International Community (Winter Park) | Winter Park | ||
| Lake Mary Prep | Lake Mary | ||
| Orangewood Christian (Maitland) | Maitland | ||
| The Masters Academy (Oviedo) | Oviedo | ||
| Trinity Preparatory (Winter Park) | Winter Park | ||
| Class 1A - Region R6 - District D11 | |||
| School | City | ||
| Discovery (Lake Alfred) | Lake Alfred | ||
| Foundation Academy (Winter Garden) | Winter Garden | ||
| Montverde Academy | Montverde | ||
| New Dimensions (Kissimmee) | Kissimmee | ||
| Tenoroc (Lakeland) | Lakeland | ||
| Windermere Prep | Windermere | ||
| Class 1A - Region R6 - District D12 | |||
| School | City | ||
| Academy at the Lakes (Land O'Lakes) | Land O Lakes | ||
| Angeline Academy (Land O'Lakes) | Land O Lakes | ||
| Brooks DeBartolo (Tampa) | Tampa | ||
| Fort Meade | Fort Meade | ||
| Frostproof | Frostproof | ||
| McKeel (Lakeland) | Lakeland | ||
| Class 1A - Region R7 - District D13 | |||
| School | City | ||
| Avon Park | Avon Park | ||
| Bradenton Christian | Bradenton | ||
| Cardinal Mooney (Sarasota) | Sarasota | ||
| Imagine School (North Port) | North Port | ||
| Lake Placid | Lake Placid | ||
| Northside Christian (St. Petersburg) | St. Petersburg | ||
| St. Stephens (Bradenton) | Bradenton | ||