| Class 1A - Region R2 - District D5 | |||
|---|---|---|---|
| School | City | ||
| Bolles (Jacksonville) | Jacksonville | ||
| Cedar Creek Christian (Jacksonville) | Jacksonville | ||
| Episcopal (Jacksonville) | Jacksonville | ||
| Hilliard | Hilliard | ||
| Providence (Jacksonville) | Jacksonville | ||
| University Christian (Jacksonville) | Jacksonville | ||
| West Nassau (Callahan) | Callahan | ||
| Class 1A - Region R2 - District D6 | |||
| School | City | ||
| Bishop Snyder (Jacksonville) | Jacksonville | ||
| Bradford (Starke) | Starke | ||
| Branford | Branford | ||
| Eagles View (Jacksonville) | Jacksonville | ||
| Fort White | Fort White | ||
| Trinity Christian (Jacksonville) | Jacksonville | ||
| Union County (Lake Butler) | Lake Butler | ||
| Class 1A - Region R2 - District D7 | |||
| School | City | ||
| Bronson | Bronson | ||
| Cedar Key | Cedar Key | ||
| Countryside Christian (Gainesville) | Gainesville | ||
| Dixie County (Cross City) | Cross City | ||
| Oak Hall (Gainesville) | Gainesville | ||
| St. Francis (Gainesville) | Gainesville | ||
| Trenton | Trenton | ||
| Williston | Williston | ||
| Class 1A - Region R2 - District D8 | |||
| School | City | ||
| Crescent City | Crescent City | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Hawthorne | Hawthorne | ||
| Interlachen | Interlachen | ||
| Keystone Heights | Keystone Heights | ||
| Taylor (Pierson) | Pierson | ||
| Trinity Christian (Deltona) | Deltona | ||
| Class 1A - Region R3 - District D9 | |||
| School | City | ||
| Lake Mary Prep | Lake Mary | ||
| Mount Dora Christian | Mount Dora | ||
| St. John Lutheran (Ocala) | Ocala | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Umatilla | Umatilla | ||
| Wildwood | Wildwood | ||
| Class 1A - Region R3 - District D10 | |||
| School | City | ||
| Faith Christian (Orlando) | Orlando | ||
| International Community (Winter Park) | Winter Park | ||
| Lake Highland Prep (Orlando) | Orlando | ||
| Orangewood Christian (Maitland) | Maitland | ||
| The Masters Academy (Oviedo) | Oviedo | ||
| Trinity Preparatory (Winter Park) | Winter Park | ||
| Class 1A - Region R3 - District D11 | |||
| School | City | ||
| Discovery (Lake Alfred) | Lake Alfred | ||
| First Academy (Orlando) | Orlando | ||
| Foundation Academy (Winter Garden) | Winter Garden | ||
| Montverde Academy | Montverde | ||
| New Dimensions (Kissimmee) | Kissimmee | ||
| Ridgeview (Davenport) | Davenport | ||
| Windermere Prep | Windermere | ||
| Class 1A - Region R3 - District D12 | |||
| School | City | ||
| Academy at the Lakes (Land O'Lakes) | Land O Lakes | ||
| Bradenton Christian | Bradenton | ||
| Brooks DeBartolo (Tampa) | Tampa | ||
| Cardinal Mooney (Sarasota) | Sarasota | ||
| Keswick Christian (St. Petersburg) | St. Petersburg | ||
| Lakeland Christian | Lakeland | ||
| Seffner Christian | Seffner | ||
| St. Stephens (Bradenton) | Bradenton | ||
| Class 1A - Region R4 - District D13 | |||
| School | City | ||
| Avon Park | Avon Park | ||
| Fort Meade | Fort Meade | ||
| Frostproof | Frostproof | ||
| John Carroll (Fort Pierce) | Fort Pierce | ||
| Lake Placid | Lake Placid | ||
| St. Edwards (Vero Beach) | Vero Beach | ||