| Class 2A - Region R2 - District D5 | |||
|---|---|---|---|
| School | City | ||
| Baker County (Glen St. Mary) | Glen St. Mary | ||
| Clay (Green Cove Springs) | Green Cove Springs | ||
| Columbia (Lake City) | Lake City | ||
| Gainesville | Gainesville | ||
| North Marion (Citra) | Citra | ||
| Ridgeview (Orange Park) | Orange Park | ||
| Class 2A - Region R2 - District D6 | |||
| School | City | ||
| Crystal River | Crystal River | ||
| Dunnellon | Dunnellon | ||
| Eustis | Eustis | ||
| Lake Weir (Ocala) | Ocala | ||
| Leesburg | Leesburg | ||
| Mount Dora | Mount Dora | ||
| Vanguard (Ocala) | Ocala | ||
| Class 2A - Region R2 - District D7 | |||
| School | City | ||
| Bishop Moore (Orlando) | Orlando | ||
| Deltona | Deltona | ||
| Innovation (Orlando) | Orlando | ||
| Jones (Orlando) | Orlando | ||
| New Smyrna Beach | New Smyrna Beach | ||
| Pine Ridge (Deltona) | Deltona | ||
| Seabreeze (Daytona Beach) | Daytona Beach | ||
| Class 2A - Region R2 - District D8 | |||
| School | City | ||
| Central (Brooksville) | Brooksville | ||
| Citrus (Inverness) | Inverness | ||
| Hernando (Brooksville) | Brooksville | ||
| Lecanto | Lecanto | ||
| Nature Coast (Brooksville) | Brooksville | ||
| Weeki Wachee | Weeki Wachee | ||
| Class 2A - Region R3 - District D9 | |||
| School | City | ||
| Clearwater | Clearwater | ||
| Countryside (Clearwater) | Clearwater | ||
| Fivay (Hudson) | Hudson | ||
| Gulf (New Port Richey) | New Port Richey | ||
| Pasco (Dade City) | Dade City | ||
| River Ridge (New Port Richey) | New Port Richey | ||
| Class 2A - Region R3 - District D10 | |||
| School | City | ||
| Brandon | Brandon | ||
| Freedom (Tampa) | Tampa | ||
| Gateway (Kissimmee) | Kissimmee | ||
| King (Tampa) | Tampa | ||
| Lake Region (Eagle Lake) | Eagle Lake | ||
| Liberty (Kissimmee) | Kissimmee | ||
| Zephyrhills | Zephyrhills | ||
| Class 2A - Region R3 - District D11 | |||
| School | City | ||
| Blake (Tampa) | Tampa | ||
| Chamberlain (Tampa) | Tampa | ||
| Hillsborough (Tampa) | Tampa | ||
| Jefferson (Tampa) | Tampa | ||
| Middleton (Tampa) | Tampa | ||
| Robinson (Tampa) | Tampa | ||
| Class 2A - Region R3 - District D12 | |||
| School | City | ||
| Bayshore (Bradenton) | Bradenton | ||
| Booker (Sarasota) | Sarasota | ||
| Northeast (St. Petersburg) | St. Petersburg | ||
| Osceola (Seminole) | Seminole | ||
| Seminole (Seminole) | Seminole | ||
| Southeast (Bradenton) | Bradenton | ||
| St. Petersburg | St. Petersburg | ||
| Class 2A - Region R4 - District D13 | |||
| School | City | ||
| Bishop Verot (Fort Myers) | Fort Myers | ||
| Bonita Springs | Bonita Springs | ||
| Cypress Lake (Fort Myers) | Fort Myers | ||
| Dunbar (Fort Myers) | Fort Myers | ||
| Estero | Estero | ||
| Island Coast (Cape Coral) | Cape Coral | ||
| South Fort Myers | Fort Myers | ||