| Class 2A - Region R2 - District D4 | |||
|---|---|---|---|
| School | City | ||
| Bishop Kenny (Jacksonville) | Jacksonville | ||
| Bolles (Jacksonville) | Jacksonville | ||
| Menendez (St. Augustine) | St. Augustine | ||
| Palatka | Palatka | ||
| Riverside (Jacksonville) | Jacksonville | ||
| Terry Parker (Jacksonville) | Jacksonville | ||
| Westside (Jacksonville) | Jacksonville | ||
| Wolfson (Jacksonville) | Jacksonville | ||
| Class 2A - Region R3 - District D5 | |||
| School | City | ||
| Bishop Moore (Orlando) | Orlando | ||
| Dunnellon | Dunnellon | ||
| Eustis | Eustis | ||
| Lake Highland Prep (Orlando) | Orlando | ||
| Lake Weir (Ocala) | Ocala | ||
| Mount Dora | Mount Dora | ||
| North Marion (Citra) | Citra | ||
| Tavares | Tavares | ||
| Umatilla | Umatilla | ||
| Villages | Middleton | ||
| Class 2A - Region R3 - District D6 | |||
| School | City | ||
| Astronaut (Titusville) | Titusville | ||
| Cocoa | Cocoa | ||
| Eau Gallie (Melbourne) | Melbourne | ||
| Merritt Island | Merritt Island | ||
| Palm Bay (Melbourne) | Melbourne | ||
| Rockledge | Rockledge | ||
| Satellite (Satellite Beach) | Satellite Beach | ||
| Space Coast (Cocoa) | Cocoa | ||
| Titusville | Titusville | ||
| Class 2A - Region R4 - District D7 | |||
| School | City | ||
| Anclote (Holiday) | Holiday | ||
| Angeline Academy (Land O'Lakes) | Land O Lakes | ||
| Angeline Academy (Land O'Lakes) | Land O Lakes | ||
| Central (Brooksville) | Brooksville | ||
| Citrus (Inverness) | Inverness | ||
| Crystal River | Crystal River | ||
| Gulf (New Port Richey) | New Port Richey | ||
| Hernando (Brooksville) | Brooksville | ||
| Hudson | Hudson | ||
| Nature Coast (Brooksville) | Brooksville | ||
| Weeki Wachee | Weeki Wachee | ||
| Class 2A - Region R4 - District D8 | |||
| School | City | ||
| Brandon | Brandon | ||
| Chamberlain (Tampa) | Tampa | ||
| Holy Names (Tampa) | Tampa | ||
| Jefferson (Tampa) | Tampa | ||
| King (Tampa) | Tampa | ||
| McKeel (Lakeland) | Lakeland | ||
| Middleton (Tampa) | Tampa | ||
| Mulberry | Mulberry | ||
| Class 2A - Region R5 - District D9 | |||
| School | City | ||
| Boca Ciega (Gulfport) | Gulfport | ||
| Calvary Christian (Clearwater) | Clearwater | ||
| Countryside (Clearwater) | Clearwater | ||
| Dunedin | Dunedin | ||
| Gibbs (St. Petersburg) | St. Petersburg | ||
| Lakewood (St. Petersburg) | St. Petersburg | ||
| Robinson (Tampa) | Tampa | ||
| Seminole (Seminole) | Seminole | ||
| Tarpon Springs | Tarpon Springs | ||
| Class 2A - Region R5 - District D10 | |||
| School | City | ||
| Avon Park | Avon Park | ||
| Bayshore (Bradenton) | Bradenton | ||
| Booker (Sarasota) | Sarasota | ||
| DeSoto County (Arcadia) | Arcadia | ||
| Hardee (Wauchula) | Wauchula | ||
| Lake Placid | Lake Placid | ||
| Class 2A - Region R6 - District D11 | |||
| School | City | ||
| Bishop Verot (Fort Myers) | Fort Myers | ||
| Clewiston | Clewiston | ||
| Island Coast (Cape Coral) | Cape Coral | ||
| LaBelle | LaBelle | ||
| Lemon Bay (Englewood) | Englewood | ||
| Oasis (Cape Coral) | Cape Coral | ||
| Class 2A - Region R6 - District D12 | |||
| School | City | ||
| Aubrey Rogers (Naples) | Naples | ||
| Barron Collier (Naples) | Naples | ||
| Bonita Springs | Bonita Springs | ||
| Cypress Lake (Fort Myers) | Fort Myers | ||
| Estero | Estero | ||
| Lely (Naples) | Naples | ||