| Class 5A - Region R1 - District D4 | |||
|---|---|---|---|
| School | City | ||
| Clay (Green Cove Springs) | Green Cove Springs | ||
| Mainland (Daytona Beach) | Daytona Beach | ||
| Menendez (St. Augustine) | St. Augustine | ||
| Middleburg | Middleburg | ||
| Ridgeview (Orange Park) | Orange Park | ||
| Seabreeze (Daytona Beach) | Daytona Beach | ||
| St. Augustine | St. Augustine | ||
| Class 5A - Region R2 - District D5 | |||
| School | City | ||
| Citrus (Inverness) | Inverness | ||
| Cypress Creek (Wesley Chapel) | Wesley Chapel | ||
| Fivay (Hudson) | Hudson | ||
| Gulf (New Port Richey) | New Port Richey | ||
| Lecanto | Lecanto | ||
| River Ridge (New Port Richey) | New Port Richey | ||
| Wesley Chapel | Wesley Chapel | ||
| Class 5A - Region R2 - District D6 | |||
| School | City | ||
| Auburndale | Auburndale | ||
| Davenport | Davenport | ||
| Gateway (Kissimmee) | Kissimmee | ||
| Lake Region (Eagle Lake) | Eagle Lake | ||
| Lake Wales | Lake Wales | ||
| Liberty (Kissimmee) | Kissimmee | ||
| Pasco (Dade City) | Dade City | ||
| Zephyrhills | Zephyrhills | ||
| Class 5A - Region R2 - District D7 | |||
| School | City | ||
| Deltona | Deltona | ||
| Jones (Orlando) | Orlando | ||
| Lake Buena Vista (Orlando) | Orlando | ||
| Leesburg | Leesburg | ||
| Pine Ridge (Deltona) | Deltona | ||
| Tavares | Tavares | ||
| Class 5A - Region R2 - District D8 | |||
| School | City | ||
| Bayside (Palm Bay) | Palm Bay | ||
| Eau Gallie (Melbourne) | Melbourne | ||
| Merritt Island | Merritt Island | ||
| Rockledge | Rockledge | ||
| Satellite (Satellite Beach) | Satellite Beach | ||
| Class 5A - Region R3 - District D9 | |||
| School | City | ||
| Blake (Tampa) | Tampa | ||
| Brandon | Brandon | ||
| Freedom (Tampa) | Tampa | ||
| King (Tampa) | Tampa | ||
| Middleton (Tampa) | Tampa | ||
| Spoto (Riverview) | Riverview | ||
| Class 5A - Region R3 - District D10 | |||
| School | City | ||
| Boca Ciega (Gulfport) | Gulfport | ||
| Clearwater | Clearwater | ||
| Hollins (St. Petersburg) | St. Petersburg | ||
| Northeast (St. Petersburg) | St. Petersburg | ||
| Osceola (Seminole) | Seminole | ||
| Seminole (Seminole) | Seminole | ||
| St. Petersburg | St. Petersburg | ||
| Class 5A - Region R3 - District D11 | |||
| School | City | ||
| Braden River (Bradenton) | Bradenton | ||
| Island Coast (Cape Coral) | Cape Coral | ||
| Mariner (Cape Coral) | Cape Coral | ||
| North Fort Myers | North Fort Myers | ||
| Parrish | Parrish | ||
| Port Charlotte | Port Charlotte | ||
| Southeast (Bradenton) | Bradenton | ||
| Class 5A - Region R3 - District D12 | |||
| School | City | ||
| Barron Collier (Naples) | Naples | ||
| Bonita Springs | Bonita Springs | ||
| Cypress Lake (Fort Myers) | Fort Myers | ||
| Dunbar (Fort Myers) | Fort Myers | ||
| Golden Gate (Naples) | Naples | ||
| Gulf Coast (Naples) | Naples | ||
| Lely (Naples) | Naples | ||
| Naples | Naples | ||