| Class 1A - Region R1 - District D4 | |||
|---|---|---|---|
| School | City | ||
| Hernando Christian (Brooksville) | Brooksville | ||
| Meadowbrook (Ocala) | Ocala | ||
| St. John Lutheran (Ocala) | Ocala | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Williston | Williston | ||
| Class 1A - Region R2 - District D5 | |||
| School | City | ||
| Astronaut (Titusville) | Titusville | ||
| Brevard HEAT (Palm Bay) | Melbourne | ||
| Cocoa Beach | Cocoa Beach | ||
| Edgewood (Merritt Island) | Merritt Island | ||
| Space Coast (Cocoa) | Cocoa | ||
| Class 1A - Region R2 - District D6 | |||
| School | City | ||
| Geneva (Casselberry) | Casselberry | ||
| Lake Highland Prep (Orlando) | Orlando | ||
| Orangewood Christian (Maitland) | Maitland | ||
| The Masters Academy (Oviedo) | Oviedo | ||
| Class 1A - Region R2 - District D7 | |||
| School | City | ||
| First Academy (Orlando) | Orlando | ||
| Foundation Academy (Winter Garden) | Winter Garden | ||
| Montverde Academy | Montverde | ||
| Santa Fe Catholic (Lakeland) | Lakeland | ||
| Windermere Prep | Windermere | ||
| Class 1A - Region R2 - District D8 | |||
| School | City | ||
| Bell Creek (Riverview) | Riverview | ||
| Seffner Christian | Seffner | ||
| Tampa Bay HEAT (Brandon) | Brandon | ||
| Tampa Prep | Tampa | ||
| Class 1A - Region R3 - District D9 | |||
| School | City | ||
| Berkeley Prep (Tampa) | Tampa | ||
| Bishop McLaughlin (Spring Hill) | Spring Hill | ||
| Cambridge Christian School (Tampa) | Tampa | ||
| Tampa Catholic | Tampa | ||
| Class 1A - Region R3 - District D10 | |||
| School | City | ||
| Calvary Christian (Clearwater) | Clearwater | ||
| Clearwater Central Catholic | Clearwater | ||
| Indian Rocks Christian (Largo) | Largo | ||
| Keswick Christian (St. Petersburg) | St. Petersburg | ||
| Class 1A - Region R3 - District D11 | |||
| School | City | ||
| Bradenton Christian | Bradenton | ||
| Cardinal Mooney (Sarasota) | Sarasota | ||
| Classical Academy (Sarasota) | Sarasota | ||
| Gulf Coast HEAT (Bradenton) | Sarasota | ||
| Sarasota Christian | Sarasota | ||
| St. Petersburg Catholic | St. Petersburg | ||
| Class 1A - Region R3 - District D12 | |||
| School | City | ||
| Bishop Verot (Fort Myers) | Fort Myers | ||
| Canterbury (Fort Myers) | Fort Myers | ||
| Community School (Naples) | Naples | ||
| Evangelical Christian (Fort Myers) | Fort Myers | ||
| First Baptist (Naples) | Naples | ||
| Marco Island Academy | Marco Island | ||
| Seacrest Country Day (Naples) | Naples | ||
| Southwest Florida Christian (Fort Myers) | Fort Myers | ||
| St. John Neumann (Naples) | Naples | ||