| Class 2A - Region R1 - District D3 | |||
|---|---|---|---|
| School | City | ||
| Baker County (Glen St. Mary) | Glen St. Mary | ||
| Columbia (Lake City) | Lake City | ||
| Middleburg | Middleburg | ||
| Ridgeview (Orange Park) | Orange Park | ||
| Suwannee (Live Oak) | Live Oak | ||
| Class 2A - Region R1 - District D4 | |||
| School | City | ||
| Bishop Kenny (Jacksonville) | Jacksonville | ||
| Clay (Green Cove Springs) | Green Cove Springs | ||
| Fernandina Beach | Fernandina Beach | ||
| Fleming Island | Fleming Island | ||
| Orange Park | Orange Park | ||
| Yulee | Yulee | ||
| Class 2A - Region R2 - District D5 | |||
| School | City | ||
| Beachside (St. Johns) | St. Johns | ||
| Menendez (St. Augustine) | St. Augustine | ||
| Palatka | Palatka | ||
| Ponte Vedra | Ponte Vedra | ||
| St. Augustine | St. Augustine | ||
| Class 2A - Region R2 - District D6 | |||
| School | City | ||
| Belleview | Belleview | ||
| Dunnellon | Dunnellon | ||
| Eastside (Gainesville) | Gainesville | ||
| Gainesville | Gainesville | ||
| Lake Weir (Ocala) | Ocala | ||
| North Marion (Citra) | Citra | ||
| Santa Fe (Alachua) | Alachua | ||
| Vanguard (Ocala) | Ocala | ||
| Class 2A - Region R2 - District D7 | |||
| School | City | ||
| Citrus (Inverness) | Inverness | ||
| Crystal River | Crystal River | ||
| Eustis | Eustis | ||
| Lecanto | Lecanto | ||
| Leesburg | Leesburg | ||
| Mount Dora | Mount Dora | ||
| South Sumter (Bushnell) | Bushnell | ||
| Tavares | Tavares | ||
| Villages | Middleton | ||
| Class 2A - Region R2 - District D8 | |||
| School | City | ||
| Atlantic (Port Orange) | Port Orange | ||
| Bishop Moore (Orlando) | Orlando | ||
| Deltona | Deltona | ||
| Gateway (Kissimmee) | Kissimmee | ||
| Innovation (Orlando) | Orlando | ||
| Jones (Orlando) | Orlando | ||
| Liberty (Kissimmee) | Kissimmee | ||
| New Smyrna Beach | New Smyrna Beach | ||
| Pine Ridge (Deltona) | Deltona | ||
| Seabreeze (Daytona Beach) | Daytona Beach | ||
| Class 2A - Region R3 - District D9 | |||
| School | City | ||
| Central (Brooksville) | Brooksville | ||
| Fivay (Hudson) | Hudson | ||
| Hernando (Brooksville) | Brooksville | ||
| Hudson | Hudson | ||
| Nature Coast (Brooksville) | Brooksville | ||
| Springstead (Spring Hill) | Spring Hill | ||
| Weeki Wachee | Weeki Wachee | ||
| Class 2A - Region R3 - District D10 | |||
| School | City | ||
| Anclote (Holiday) | Holiday | ||
| Angeline Academy (Land O'Lakes) | Land O Lakes | ||
| Angeline Academy (Land O'Lakes) | Land O Lakes | ||
| Cypress Creek (Wesley Chapel) | Wesley Chapel | ||
| Gulf (New Port Richey) | New Port Richey | ||
| Pasco (Dade City) | Dade City | ||
| River Ridge (New Port Richey) | New Port Richey | ||
| Wesley Chapel | Wesley Chapel | ||
| Zephyrhills | Zephyrhills | ||
| Class 2A - Region R3 - District D11 | |||
| School | City | ||
| Auburndale | Auburndale | ||
| Lake Region (Eagle Lake) | Eagle Lake | ||
| Lake Wales | Lake Wales | ||
| McKeel (Lakeland) | Lakeland | ||
| Mulberry | Mulberry | ||
| Tenoroc (Lakeland) | Lakeland | ||