| Class 2A - Region R1 - District D3 | |||
|---|---|---|---|
| School | City | ||
| Bishop Kenny (Jacksonville) | Jacksonville | ||
| Fernandina Beach | Fernandina Beach | ||
| Paxon (Jacksonville) | Jacksonville | ||
| Raines (Jacksonville) | Jacksonville | ||
| Riverside (Jacksonville) | Jacksonville | ||
| Stanton (Jacksonville) | Jacksonville | ||
| Terry Parker (Jacksonville) | Jacksonville | ||
| West Nassau (Callahan) | Callahan | ||
| Yulee | Yulee | ||
| Class 2A - Region R1 - District D4 | |||
| School | City | ||
| Clay (Green Cove Springs) | Green Cove Springs | ||
| Fleming Island | Fleming Island | ||
| Menendez (St. Augustine) | St. Augustine | ||
| Middleburg | Middleburg | ||
| Palatka | Palatka | ||
| Ridgeview (Orange Park) | Orange Park | ||
| St. Augustine | St. Augustine | ||
| Wolfson (Jacksonville) | Jacksonville | ||
| Class 2A - Region R2 - District D5 | |||
| School | City | ||
| Belleview | Belleview | ||
| Citrus (Inverness) | Inverness | ||
| Crystal River | Crystal River | ||
| Dunnellon | Dunnellon | ||
| Lake Weir (Ocala) | Ocala | ||
| Lecanto | Lecanto | ||
| Leesburg | Leesburg | ||
| North Marion (Citra) | Citra | ||
| South Sumter (Bushnell) | Bushnell | ||
| Vanguard (Ocala) | Ocala | ||
| Villages | Middleton | ||
| Class 2A - Region R2 - District D6 | |||
| School | City | ||
| Atlantic (Port Orange) | Port Orange | ||
| Bishop Moore (Orlando) | Orlando | ||
| Deltona | Deltona | ||
| Innovation (Orlando) | Orlando | ||
| Mount Dora | Mount Dora | ||
| New Smyrna Beach | New Smyrna Beach | ||
| Pine Ridge (Deltona) | Deltona | ||
| Seabreeze (Daytona Beach) | Daytona Beach | ||
| Tavares | Tavares | ||
| Umatilla | Umatilla | ||
| Class 2A - Region R2 - District D7 | |||
| School | City | ||
| Astronaut (Titusville) | Titusville | ||
| Cocoa | Cocoa | ||
| Eau Gallie (Melbourne) | Melbourne | ||
| Merritt Island | Merritt Island | ||
| Palm Bay (Melbourne) | Melbourne | ||
| Rockledge | Rockledge | ||
| Satellite (Satellite Beach) | Satellite Beach | ||
| Sebastian River (Sebastian) | Sebastian | ||
| Titusville | Titusville | ||
| Class 2A - Region R2 - District D8 | |||
| School | City | ||
| Angeline Academy (Land O'Lakes) | Land O Lakes | ||
| Central (Brooksville) | Brooksville | ||
| Cypress Creek (Wesley Chapel) | Wesley Chapel | ||
| Hernando (Brooksville) | Brooksville | ||
| Hudson | Hudson | ||
| Nature Coast (Brooksville) | Brooksville | ||
| Pasco (Dade City) | Dade City | ||
| Springstead (Spring Hill) | Spring Hill | ||
| Weeki Wachee | Weeki Wachee | ||
| Wesley Chapel | Wesley Chapel | ||
| Zephyrhills | Zephyrhills | ||
| Class 2A - Region R3 - District D9 | |||
| School | City | ||
| Auburndale | Auburndale | ||
| Avon Park | Avon Park | ||
| DeSoto County (Arcadia) | Arcadia | ||
| Hardee (Wauchula) | Wauchula | ||
| Lake Wales | Lake Wales | ||
| McKeel (Lakeland) | Lakeland | ||
| Mulberry | Mulberry | ||
| Class 2A - Region R3 - District D10 | |||
| School | City | ||
| Blake (Tampa) | Tampa | ||
| Brandon | Brandon | ||
| Chamberlain (Tampa) | Tampa | ||
| East Bay (Gibsonton) | Gibsonton | ||
| Freedom (Tampa) | Tampa | ||
| Hillsborough (Tampa) | Tampa | ||
| Holy Names (Tampa) | Tampa | ||
| Jefferson (Tampa) | Tampa | ||
| King (Tampa) | Tampa | ||
| Middleton (Tampa) | Tampa | ||
| Morgan (Wimauma) | Wimauma | ||
| Robinson (Tampa) | Tampa | ||
| Class 2A - Region R3 - District D11 | |||
| School | City | ||
| Anclote (Holiday) | Holiday | ||
| Clearwater | Clearwater | ||
| Countryside (Clearwater) | Clearwater | ||
| Dunedin | Dunedin | ||
| Osceola (Seminole) | Seminole | ||
| Pinellas Park (Largo) | Largo | ||
| River Ridge (New Port Richey) | New Port Richey | ||
| Seminole (Seminole) | Seminole | ||
| Tarpon Springs | Tarpon Springs | ||