| Class 1A - Region R1 - District D4 | |||
|---|---|---|---|
| School | City | ||
| Countryside Christian (Gainesville) | Gainesville | ||
| Redeemer Christian (Ocala) | Ocala | ||
| Seven Rivers Christian (Lecanto) | Lecanto | ||
| St. John Lutheran (Ocala) | Ocala | ||
| Class 1A - Region R2 - District D5 | |||
| School | City | ||
| Academy at the Lakes (Land O'Lakes) | Land O Lakes | ||
| Classical Prep (Shady Hills) | Spring Hill | ||
| Hernando Christian (Brooksville) | Brooksville | ||
| North Tampa Christian (Wesley Chapel) | Wesley Chapel | ||
| Class 1A - Region R2 - District D6 | |||
| School | City | ||
| Calvary Christian (Ormond Beach) | Ormond Beach | ||
| First Baptist (Palm Coast) | Palm Coast | ||
| Halifax Academy (Daytona Beach) | Daytona Beach | ||
| Mount Dora Christian | Mount Dora | ||
| Trinity Christian (Deltona) | Deltona | ||
| Class 1A - Region R2 - District D7 | |||
| School | City | ||
| Altamonte Christian (Altamonte Springs) | Altamonte Springs | ||
| Central Florida Christian (Orlando) | Orlando | ||
| Holy Cross (Sanford) | Sanford | ||
| Lake Mary Prep | Lake Mary | ||
| Legacy (Ocoee) | Ocoee | ||
| Orlando Christian Prep | Orlando | ||
| Class 1A - Region R2 - District D8 | |||
| School | City | ||
| Calvary Chapel (West Melbourne) | West Melbourne | ||
| Masters Academy (Vero Beach) | Vero Beach | ||
| Merritt Island Christian | Merritt Island | ||
| Morningside (Port St. Lucie) | Port St. Lucie | ||
| St. Edwards (Vero Beach) | Vero Beach | ||
| Class 1A - Region R3 - District D9 | |||
| School | City | ||
| All Saints' (Winter Haven) | Winter Haven | ||
| City of Life Christian (Kissimmee) | Kissimmee | ||
| Oasis Christian (Winter Haven) | Winter Haven | ||
| Victory Christian (Lakeland) | Lakeland | ||
| Class 1A - Region R3 - District D10 | |||
| School | City | ||
| Bayshore Christian (Tampa) | Tampa | ||
| Cambridge Christian School (Tampa) | Tampa | ||
| Foundation Christian (Valrico) | Valrico | ||
| Universal Academy (Tampa) | Tampa | ||
| Class 1A - Region R3 - District D11 | |||
| School | City | ||
| Keswick Christian (St. Petersburg) | St. Petersburg | ||
| Lakeside Christian (Clearwater) | Clearwater | ||
| Sarasota Christian | Sarasota | ||
| Class 1A - Region R3 - District D12 | |||
| School | City | ||
| Babcock (Babcock Ranch) | Babcock Ranch | ||
| Donahue Academy (Ave Maria) | Ave Maria | ||
| Everglades City | Everglades City | ||
| Seacrest Country Day (Naples) | Naples | ||
| Southwest Florida Christian (Fort Myers) | Fort Myers | ||