| Class 1A - Region R1 - District D3 | |||
|---|---|---|---|
| School | City | ||
| Impact Christian (Jacksonville) | Jacksonville | ||
| Providence (Jacksonville) | Jacksonville | ||
| University Christian (Jacksonville) | Jacksonville | ||
| Class 1A - Region R1 - District D4 | |||
| School | City | ||
| P. K. Yonge (Gainesville) | Gainesville | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Class 1A - Region R2 - District D5 | |||
| School | City | ||
| Crescent City | Crescent City | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Class 1A - Region R2 - District D6 | |||
| School | City | ||
| First Academy (Orlando) | Orlando | ||
| Melbourne Central Catholic | Melbourne | ||
| Orlando Christian Prep | Orlando | ||
| Class 1A - Region R2 - District D7 | |||
| School | City | ||
| Carrollwood (Tampa) | Tampa | ||
| Lakeland Christian | Lakeland | ||
| Victory Christian (Lakeland) | Lakeland | ||
| Class 1A - Region R2 - District D8 | |||
| School | City | ||
| Clearwater Central Catholic | Clearwater | ||
| Indian Rocks Christian (Largo) | Largo | ||
| Class 1A - Region R3 - District D9 | |||
| School | City | ||
| Northside Christian (St. Petersburg) | St. Petersburg | ||
| St. Petersburg Catholic | St. Petersburg | ||
| Class 1A - Region R3 - District D10 | |||
| School | City | ||
| Evangelical Christian (Fort Myers) | Fort Myers | ||
| Southwest Florida Christian (Fort Myers) | Fort Myers | ||
| Class 1A - Region R3 - District D11 | |||
| School | City | ||
| Community School (Naples) | Naples | ||
| First Baptist (Naples) | Naples | ||
| St. John Neumann (Naples) | Naples | ||