| Class 2A - Region R1 - District D3 | |||
|---|---|---|---|
| School | City | ||
| Bishop Snyder (Jacksonville) | Jacksonville | ||
| Bolles (Jacksonville) | Jacksonville | ||
| Episcopal (Jacksonville) | Jacksonville | ||
| Providence (Jacksonville) | Jacksonville | ||
| Trinity Christian (Jacksonville) | Jacksonville | ||
| Class 2A - Region R1 - District D4 | |||
| School | City | ||
| Crescent City | Crescent City | ||
| Interlachen | Interlachen | ||
| Keystone Heights | Keystone Heights | ||
| Newberry | Newberry | ||
| P. K. Yonge (Gainesville) | Gainesville | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Class 2A - Region R2 - District D5 | |||
| School | City | ||
| Crooms (Sanford) | Sanford | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Lake Highland Prep (Orlando) | Orlando | ||
| Taylor (Pierson) | Pierson | ||
| The Masters Academy (Oviedo) | Oviedo | ||
| Trinity Preparatory (Winter Park) | Winter Park | ||
| Class 2A - Region R2 - District D6 | |||
| School | City | ||
| Cornerstone Charter (Belle Isle) | Belle Isle | ||
| First Academy (Orlando) | Orlando | ||
| Foundation Academy (Winter Garden) | Winter Garden | ||
| Montverde Academy | Montverde | ||
| Windermere Prep | Windermere | ||
| Class 2A - Region R2 - District D7 | |||
| School | City | ||
| Discovery (Lake Alfred) | Lake Alfred | ||
| Lakeland Christian | Lakeland | ||
| Santa Fe Catholic (Lakeland) | Lakeland | ||
| Class 2A - Region R2 - District D8 | |||
| School | City | ||
| Cocoa Beach | Cocoa Beach | ||
| Holy Trinity (Melbourne) | Melbourne | ||
| John Carroll (Fort Pierce) | Fort Pierce | ||
| Melbourne Central Catholic | Melbourne | ||
| Class 2A - Region R3 - District D9 | |||
| School | City | ||
| Berkeley Prep (Tampa) | Tampa | ||
| Bishop McLaughlin (Spring Hill) | Spring Hill | ||
| Brooks DeBartolo (Tampa) | Tampa | ||
| Carrollwood (Tampa) | Tampa | ||
| Tampa Catholic | Tampa | ||
| Class 2A - Region R3 - District D10 | |||
| School | City | ||
| Bell Creek (Riverview) | Riverview | ||
| Clearwater Central Catholic | Clearwater | ||
| Shorecrest Prep (St. Petersburg) | St. Petersburg | ||
| St. Petersburg Catholic | St. Petersburg | ||
| Tampa Prep | Tampa | ||
| Class 2A - Region R3 - District D11 | |||
| School | City | ||
| Cardinal Mooney (Sarasota) | Sarasota | ||
| Imagine School (North Port) | North Port | ||
| Out-of-Door (Sarasota) | Sarasota | ||
| Sarasota Military Academy | Sarasota | ||