| Class 2A - Region R1 - District D3 | |||
|---|---|---|---|
| School | City | ||
| Baldwin | Baldwin | ||
| Bishop Snyder (Jacksonville) | Jacksonville | ||
| Bradford (Starke) | Starke | ||
| Trinity Christian (Jacksonville) | Jacksonville | ||
| Class 2A - Region R1 - District D4 | |||
| School | City | ||
| Andrew Jackson (Jacksonville) | Jacksonville | ||
| Bolles (Jacksonville) | Jacksonville | ||
| Episcopal (Jacksonville) | Jacksonville | ||
| Providence (Jacksonville) | Jacksonville | ||
| Class 2A - Region R2 - District D5 | |||
| School | City | ||
| Interlachen | Interlachen | ||
| Keystone Heights | Keystone Heights | ||
| Newberry | Newberry | ||
| P. K. Yonge (Gainesville) | Gainesville | ||
| Class 2A - Region R2 - District D6 | |||
| School | City | ||
| Crescent City | Crescent City | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Taylor (Pierson) | Pierson | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Umatilla | Umatilla | ||
| Class 2A - Region R2 - District D7 | |||
| School | City | ||
| Cornerstone Charter (Belle Isle) | Belle Isle | ||
| First Academy (Orlando) | Orlando | ||
| Lake Highland Prep (Orlando) | Orlando | ||
| Montverde Academy | Montverde | ||
| Windermere Prep | Windermere | ||
| Class 2A - Region R2 - District D8 | |||
| School | City | ||
| Cocoa Beach | Cocoa Beach | ||
| Melbourne Central Catholic | Melbourne | ||
| Space Coast (Cocoa) | Cocoa | ||
| Trinity Preparatory (Winter Park) | Winter Park | ||
| Class 2A - Region R3 - District D9 | |||
| School | City | ||
| Berkeley Prep (Tampa) | Tampa | ||
| Carrollwood (Tampa) | Tampa | ||
| Tampa Catholic | Tampa | ||
| Class 2A - Region R3 - District D10 | |||
| School | City | ||
| Avon Park | Avon Park | ||
| Discovery (Lake Alfred) | Lake Alfred | ||
| Lake Placid | Lake Placid | ||
| Class 2A - Region R3 - District D11 | |||
| School | City | ||
| Calvary Christian (Clearwater) | Clearwater | ||
| Cardinal Mooney (Sarasota) | Sarasota | ||
| Clearwater Central Catholic | Clearwater | ||
| Lakewood (St. Petersburg) | St. Petersburg | ||
| St. Petersburg Catholic | St. Petersburg | ||