| Class 2A - Region R1 - District D3 | |||
|---|---|---|---|
| School | City | ||
| Circle Christian (Winter Park) | Winter Springs | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Orangewood Christian (Maitland) | Maitland | ||
| The Masters Academy (Oviedo) | Oviedo | ||
| Trinity Preparatory (Winter Park) | Winter Park | ||
| Class 2A - Region R1 - District D4 | |||
| School | City | ||
| Cornerstone Charter (Belle Isle) | Belle Isle | ||
| Faith Christian (Orlando) | Orlando | ||
| First Academy (Orlando) | Orlando | ||
| Foundation Academy (Winter Garden) | Winter Garden | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Class 2A - Region R2 - District D5 | |||
| School | City | ||
| Lakeland Christian | Lakeland | ||
| New Dimensions (Kissimmee) | Kissimmee | ||
| Ridgeview (Davenport) | Davenport | ||
| Santa Fe Catholic (Lakeland) | Lakeland | ||
| Seffner Christian | Seffner | ||
| Tampa Bay HEAT (Brandon) | Brandon | ||
| Class 2A - Region R2 - District D6 | |||
| School | City | ||
| Bell Creek (Riverview) | Riverview | ||
| Bishop McLaughlin (Spring Hill) | Spring Hill | ||
| Cambridge Christian School (Tampa) | Tampa | ||
| Carrollwood (Tampa) | Tampa | ||
| SLAM (Tampa) | Tampa | ||
| Tampa Prep | Tampa | ||
| Class 2A - Region R2 - District D7 | |||
| School | City | ||
| Admiral Farragut (St. Petersburg) | St. Petersburg | ||
| Clearwater Central Catholic | Clearwater | ||
| Indian Rocks Christian (Largo) | Largo | ||
| Northside Christian (St. Petersburg) | St. Petersburg | ||
| Shorecrest Prep (St. Petersburg) | St. Petersburg | ||
| St. Petersburg Catholic | St. Petersburg | ||
| Class 2A - Region R2 - District D8 | |||
| School | City | ||
| Bradenton Christian | Bradenton | ||
| Imagine School (North Port) | North Port | ||
| Out-of-Door (Sarasota) | Sarasota | ||
| St. Stephens (Bradenton) | Bradenton | ||
| Class 2A - Region R3 - District D9 | |||
| School | City | ||
| Canterbury (Fort Myers) | Fort Myers | ||
| Community School (Naples) | Naples | ||
| Evangelical Christian (Fort Myers) | Fort Myers | ||
| First Baptist (Naples) | Naples | ||
| Marco Island Academy | Marco Island | ||
| Mason Classical Academy (Naples) | Naples | ||
| Southwest Florida Christian (Fort Myers) | Fort Myers | ||
| St. John Neumann (Naples) | Naples | ||
| Class 2A - Region R3 - District D10 | |||
| School | City | ||
| Edgewood (Merritt Island) | Merritt Island | ||
| Holy Trinity (Melbourne) | Melbourne | ||
| Melbourne Central Catholic | Melbourne | ||
| Odyssey Charter (Palm Bay) | Palm Bay | ||
| Pineapple Cove (Palm Bay) | Palm Bay | ||
| West Shore (Melbourne) | Melbourne | ||
| Class 2A - Region R3 - District D11 | |||
| School | City | ||
| Benjamin (Palm Beach Gardens) | Palm Beach Gardens | ||
| Jupiter Christian | Jupiter | ||
| Oxbridge Academy (West Palm Beach) | West Palm Beach | ||