| Class 2A - Region R1 - District D2 | |||
|---|---|---|---|
| School | City | ||
| Bell | Bell | ||
| Branford | Branford | ||
| Fort White | Fort White | ||
| Hamilton County (Jasper) | Jasper | ||
| Lafayette (Mayo) | Mayo | ||
| Class 2A - Region R1 - District D3 | |||
| School | City | ||
| Newberry | Newberry | ||
| P. K. Yonge (Gainesville) | Gainesville | ||
| Trenton | Trenton | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Williston | Williston | ||
| Class 2A - Region R1 - District D4 | |||
| School | City | ||
| Bishop Snyder (Jacksonville) | Jacksonville | ||
| Episcopal (Jacksonville) | Jacksonville | ||
| Global Academy | Jacksonville | ||
| Providence (Jacksonville) | Jacksonville | ||
| Trinity Christian (Jacksonville) | Jacksonville | ||
| Class 2A - Region R2 - District D5 | |||
| School | City | ||
| Crescent City | Crescent City | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Taylor (Pierson) | Pierson | ||
| The Masters Academy (Oviedo) | Oviedo | ||
| Trinity Preparatory (Winter Park) | Winter Park | ||
| Class 2A - Region R2 - District D6 | |||
| School | City | ||
| Cornerstone Charter (Belle Isle) | Belle Isle | ||
| First Academy (Orlando) | Orlando | ||
| Foundation Academy (Winter Garden) | Winter Garden | ||
| Windermere Prep | Windermere | ||
| Class 2A - Region R2 - District D7 | |||
| School | City | ||
| Edgewood (Merritt Island) | Merritt Island | ||
| Holy Trinity (Melbourne) | Melbourne | ||
| John Carroll (Fort Pierce) | Fort Pierce | ||
| Melbourne Central Catholic | Melbourne | ||
| Somerset College Prep (Port St Lucie) | Port St. Lucie | ||
| West Shore (Melbourne) | Melbourne | ||
| Class 2A - Region R2 - District D8 | |||
| School | City | ||
| Benjamin (Palm Beach Gardens) | Palm Beach Gardens | ||
| Cardinal Newman (West Palm Beach) | West Palm Beach | ||
| Kings Academy (West Palm Beach) | West Palm Beach | ||
| Oxbridge Academy (West Palm Beach) | West Palm Beach | ||
| Pahokee | Pahokee | ||
| Class 2A - Region R3 - District D9 | |||
| School | City | ||
| Fort Meade | Fort Meade | ||
| Frostproof | Frostproof | ||
| Lakeland Christian | Lakeland | ||
| Ridgeview (Davenport) | Davenport | ||
| Santa Fe Catholic (Lakeland) | Lakeland | ||
| Class 2A - Region R3 - District D10 | |||
| School | City | ||
| Bell Creek (Riverview) | Riverview | ||
| Bishop McLaughlin (Spring Hill) | Spring Hill | ||
| Brooks DeBartolo (Tampa) | Tampa | ||
| Carrollwood (Tampa) | Tampa | ||
| Tampa Prep | Tampa | ||