| Class 2A - Region R1 - District D1 | |||
|---|---|---|---|
| School | City | ||
| Community Christian (Tallahassee) | Tallahassee | ||
| FAMU (Tallahassee) | Tallahassee | ||
| LEAD Academy (Pace) | Pace | ||
| Munroe (Quincy) | Quincy | ||
| North Florida Christian (Tallahassee) | Tallahassee | ||
| St. John Paul II (Tallahassee) | Tallahassee | ||
| Class 2A - Region R1 - District D2 | |||
| School | City | ||
| Christs Church Academy (Jacksonville) | Jacksonville | ||
| Eagles View (Jacksonville) | Jacksonville | ||
| First Coast Christian (Jacksonville) | Jacksonville | ||
| St. Johns Country Day (Orange Park) | Orange Park | ||
| University Christian (Jacksonville) | Jacksonville | ||
| Class 2A - Region R1 - District D3 | |||
| School | City | ||
| Beaches Chapel (Neptune Beach) | Neptune Beach | ||
| Covenant School (Jacksonville) | Jacksonville | ||
| Harvest Community (Jacksonville) | Jacksonville | ||
| Parsons Christian (Jacksonville) | Jacksonville | ||
| Class 2A - Region R1 - District D4 | |||
| School | City | ||
| Countryside Christian (Gainesville) | Gainesville | ||
| Florida Deaf and Blind (St. Augustine) | St. Augustine | ||
| Oak Hall (Gainesville) | Gainesville | ||
| Peniel Baptist (Palatka) | Palatka | ||
| St. Francis (Gainesville) | Gainesville | ||
| St. Joseph (St. Augustine) | St. Augustine | ||
| Class 2A - Region R2 - District D5 | |||
| School | City | ||
| Calvary Christian (Ormond Beach) | Ormond Beach | ||
| First Baptist (Palm Coast) | Palm Coast | ||
| Meadowbrook (Ocala) | Ocala | ||
| Redeemer Christian (Ocala) | Ocala | ||
| St. John Lutheran (Ocala) | Ocala | ||
| Trinity Christian (Deltona) | Deltona | ||
| Class 2A - Region R2 - District D6 | |||
| School | City | ||
| First Academy-Leesburg | Leesburg | ||
| Geneva (Casselberry) | Casselberry | ||
| International Community (Winter Park) | Winter Park | ||
| Lake Mary Prep | Lake Mary | ||
| Mount Dora Christian | Mount Dora | ||
| Orangewood Christian (Maitland) | Maitland | ||
| Class 2A - Region R2 - District D7 | |||
| School | City | ||
| Central Florida Christian (Orlando) | Orlando | ||
| Central Florida Leadership (Orlando) | Orlando | ||
| City of Life Christian (Kissimmee) | Kissimmee | ||
| Faith Christian (Orlando) | Orlando | ||
| Legacy (Ocoee) | Ocoee | ||
| Orlando Christian Prep | Orlando | ||
| Class 2A - Region R2 - District D8 | |||
| School | City | ||
| Brevard HEAT (Palm Bay) | Melbourne | ||
| Masters Academy (Vero Beach) | Vero Beach | ||
| Merritt Island Christian | Merritt Island | ||
| Morningside (Port St. Lucie) | Port St. Lucie | ||
| Pineapple Cove (Palm Bay) | Palm Bay | ||
| St. Edwards (Vero Beach) | Vero Beach | ||
| Class 2A - Region R3 - District D9 | |||
| School | City | ||
| Academy at the Lakes (Land O'Lakes) | Land O Lakes | ||
| Cambridge Christian School (Tampa) | Tampa | ||
| Classical Prep (Shady Hills) | Spring Hill | ||
| Hernando Christian (Brooksville) | Brooksville | ||
| North Tampa Christian (Wesley Chapel) | Wesley Chapel | ||
| Seven Rivers Christian (Lecanto) | Lecanto | ||
| Universal Academy (Tampa) | Tampa | ||
| Victory Christian (Lakeland) | Lakeland | ||