| Class 4A - Region R1 - District D1 | |||
|---|---|---|---|
| School | City | ||
| Atlantic Coast (Jacksonville) | Jacksonville | ||
| Mandarin (Jacksonville) | Jacksonville | ||
| Oakleaf (Orange Park) | Orange Park | ||
| Sandalwood (Jacksonville) | Jacksonville | ||
| Tocoi Creek (St. Augustine) | St. Augustine | ||
| Class 4A - Region R1 - District D2 | |||
| School | City | ||
| DeLand | DeLand | ||
| Flagler Palm Coast | Palm Coast | ||
| Forest (Ocala) | Ocala | ||
| Spruce Creek (Port Orange) | Port Orange | ||
| University (Orange City) | Orange City | ||
| West Port (Ocala) | Ocala | ||
| Class 4A - Region R1 - District D3 | |||
| School | City | ||
| Apopka | Apopka | ||
| Evans (Orlando) | Orlando | ||
| Lake Brantley | Altamonte Springs | ||
| Lake Mary | Lake Mary | ||
| Ocoee | Ocoee | ||
| Seminole (Sanford) | Sanford | ||
| Class 4A - Region R1 - District D4 | |||
| School | City | ||
| Boone (Orlando) | Orlando | ||
| Colonial (Orlando) | Orlando | ||
| Hagerty (Oviedo) | Oviedo | ||
| Timber Creek (Orlando) | Orlando | ||
| University (Orlando) | Orlando | ||
| Winter Park | Winter Park | ||
| Class 4A - Region R2 - District D5 | |||
| School | City | ||
| Dr. Phillips (Orlando) | Orlando | ||
| East Ridge (Clermont) | Clermont | ||
| Horizon (Winter Garden) | Winter Garden | ||
| Olympia (Orlando) | Orlando | ||
| West Orange (Winter Garden) | Winter Garden | ||
| Windermere | Windermere | ||
| Class 4A - Region R2 - District D6 | |||
| School | City | ||
| Durant (Plant City) | Plant City | ||
| George Jenkins (Lakeland) | Lakeland | ||
| Haines City | Haines City | ||
| Plant City | Plant City | ||
| Ridge Community (Davenport) | Haines City | ||
| Winter Haven | Winter Haven | ||
| Class 4A - Region R2 - District D7 | |||
| School | City | ||
| Alonso (Tampa) | Tampa | ||
| Armwood (Seffner) | Seffner | ||
| George Steinbrenner (Lutz) | Lutz | ||
| Palm Harbor University | Palm Harbor | ||
| Plant (Tampa) | Tampa | ||
| Sickles (Tampa) | Tampa | ||
| Strawberry Crest (Dover) | Dover | ||
| Class 4A - Region R2 - District D8 | |||
| School | City | ||
| Bloomingdale (Valrico) | Valrico | ||
| Lakewood Ranch (Bradenton) | Bradenton | ||
| Lennard (Ruskin) | Ruskin | ||
| Newsome (Lithia) | Lithia | ||
| Riverview (Riverview) | Riverview | ||
| Sumner (Riverview) | Riverview | ||
| Class 4A - Region R3 - District D9 | |||
| School | City | ||
| Cypress Creek (Orlando) | Orlando | ||
| Freedom (Orlando) | Orlando | ||
| Lake Nona (Orlando) | Orlando | ||
| Oak Ridge (Orlando) | Orlando | ||
| Tohopekaliga (Kissimmee) | Kissimmee | ||